Could you suggest from where can I find the EEG data set of patients with Disorder of Consciousness?

I am a research scholar working in brain computer interface for DOC patients. I am looking for EEG data set specifically of the patients with consciousness disorder. Could you suggest any references?

25 August 2020 3,969 2 View

Whats the optimum dose of TNFa neutralizing Ab for an invivo study?

Has anyone used any of the mouse TNFa neutralizing antibody in vivo? Whats the ideal dose that i could possibly use?

09 September 2019 5,765 1 View

Dear friends, can I run SEM for each pair of constructs separately or single SEM for all constructs simultaneously?

Dear friends, My model includes 6 constructs. 2 independent constructs, one dependent construct and 3 mediators. My hypotheses include the relationship between each construct and the mediating...

06 April 2019 6,716 5 View

Dear friends, can I run SEM for each pair of constructs separately or single SEM for all constructs simultaneously?

Dear friends, My model includes 6 constructs. 2 independent constructs, one dependent construct and 3 mediators. My hypotheses include the relationship between each construct and the mediating...

06 April 2019 2,117 5 View

Using pCMV-eGFP as an empty control for pCMV-ProteinX-GFP?

I have a really old construct that contains GFP (original) tagged protein under CMV promoter. I can't find an empty vector containing GFP (Addgene or similar). The closest they have is eGFP under...

10 March 2019 6,929 3 View

Why Slow freezing and Fast thawing in Cryopreservation of mammalian cells?

(a) What is the rationale of doing a slow freezing (controlled rate freezing)? (b) Why a quick thawing of frozen vials at 37 degree C? I read some research articles , but confused about...

16 December 2017 4,099 8 View

What defines the age of a primary cell line?

I am using some primary human cells for my experiments, But the activity/proliferation rate of the cells in their late passages (>P8) are very low and I can not use them for long like cell...

16 December 2017 4,027 3 View

How to find CNV from RADseq data?

How to find Copy Number Variations from RAD/ddRAD sequencing data. Is there any software available for the same

29 August 2017 5,315 3 View

Can anybody suggest me a list of wireless card drivers that support OCB(Outside the context of a basic station set) mode ?

I have tried out the ath9k driver with an appropriate atheros card. But while setting the card to ocb mode I recieved an error.These are the commands I used. sudo iw reg set DEsudo ip link set...

17 October 2016 7,219 3 View

Why only a meger OCV of 200mV is observed with graphene used as anode in MFC?

I am getting very little OCV with grapehen anode . However a very good OCV was obtained with graphite anode. Please help!!

25 May 2016 7,959 1 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View