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Questions related from Anirudha Sahu
Hi One of my friend had kept 2 ml of filtered incomplete DMEM media for check for 6 days in a 35 mm UV sterilized petri plate (Kept in UV for 1 hour). He had also checked before keeping it in the...
28 February 2020 713 0 View
I am working on a drug called Temozolomide in my cell culture experiments. I am having trouble in preparing working concentrations of this drug from 200 to 2000 uM as the drug is soluble in DMSO...
16 July 2019 4,384 3 View
I am planning to order antibodies for my experiment which can be used for ChIP, Western blot, Immuno-fluorescence (IF) and Immuno-histochemistry. Some suppliers mention their product to be...
13 February 2018 5,022 4 View
Please help me find out if stress induced during in planta transformation in any crop may lead to somaclonal variations. Please give some references. It's urgent. Thank you
08 August 2017 6,970 4 View
Just curious if there can be somaclonal variation in direct organogenesis or not; because in direct organogenesis i.e. from explant to shoot and then root occurs directly without the intermediate...
06 August 2017 2,079 4 View
I talked with one of my professor; he told that chimeric plants are mostly found in T0 generation only and the opportunity for finding a chimeric T1 generation plant is extremely rare or most of...
13 June 2017 4,139 25 View
I am doing a 12% SDS PAGE gel after total plant protein extraction from cotton leaf by TCA/ACETONE precipitation method. I am loading the crude protein without dilution after keeping it for...
28 April 2017 831 21 View
I want a total plant protein extraction protocol specific for cotton leaf to do SDS-PAGE of the extracted total plant protein. what should be the pH of the extraction buffer? I want the proteins...
27 February 2017 7,733 3 View
In my lectin haemaglutination assay by doing 2-fold serial dilution I have to add 50ul of trypsinized rabbit erythrocytes instead of normal rabbit erythrocytes and also I have heard that the wells...
04 October 2016 2,491 1 View
What I have heard is the normal Taq Polymerase which we commonly use can amplify a fragment upto 1.5 kb. But yesterday someone told me that the normal Taq Pol can amplify a fragment upto 3kb...
23 September 2016 1,889 3 View
This method of PCR amplification produces large concatamers of multi-looped amplicons those may show prominent visibility on the gel. But how this confers specificity of the amplification. Or if...
13 April 2016 2,488 3 View
Is it possible that combs have accumulated DNA samples from previous loadings. Because for me it seems that every time we run a gel we first remove the comb just after casting and then only run...
10 April 2016 2,349 8 View
After removing the gel solution from the microwave we keep it for cooling for nearly 10 to 15 minutes so that the temperature reduces to 55-60 degrees and then only add EtBr. Why can't we directly...
13 March 2016 5,064 3 View
