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Questions related from Anirudha Sahu
I am working on a drug called Temozolomide in my cell culture experiments. I am having trouble in preparing working concentrations of this drug from 200 to 2000 uM as the drug is soluble in DMSO...
16 July 2019 4,325 3 View
I am planning to order antibodies for my experiment which can be used for ChIP, Western blot, Immuno-fluorescence (IF) and Immuno-histochemistry. Some suppliers mention their product to be...
13 February 2018 4,973 4 View
I am doing a 12% SDS PAGE gel after total plant protein extraction from cotton leaf by TCA/ACETONE precipitation method. I am loading the crude protein without dilution after keeping it for...
28 April 2017 707 21 View
What I have heard is the normal Taq Polymerase which we commonly use can amplify a fragment upto 1.5 kb. But yesterday someone told me that the normal Taq Pol can amplify a fragment upto 3kb...
23 September 2016 1,811 3 View
This method of PCR amplification produces large concatamers of multi-looped amplicons those may show prominent visibility on the gel. But how this confers specificity of the amplification. Or if...
13 April 2016 2,428 3 View
Is it possible that combs have accumulated DNA samples from previous loadings. Because for me it seems that every time we run a gel we first remove the comb just after casting and then only run...
10 April 2016 2,300 8 View
After removing the gel solution from the microwave we keep it for cooling for nearly 10 to 15 minutes so that the temperature reduces to 55-60 degrees and then only add EtBr. Why can't we directly...
13 March 2016 5,005 3 View
