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Questions related from Anirudha Sahu
Hi One of my friend had kept 2 ml of filtered incomplete DMEM media for check for 6 days in a 35 mm UV sterilized petri plate (Kept in UV for 1 hour). He had also checked before keeping it in the...
28 February 2020 710 0 View
I am working on a drug called Temozolomide in my cell culture experiments. I am having trouble in preparing working concentrations of this drug from 200 to 2000 uM as the drug is soluble in DMSO...
16 July 2019 4,380 3 View
I am planning to order antibodies for my experiment which can be used for ChIP, Western blot, Immuno-fluorescence (IF) and Immuno-histochemistry. Some suppliers mention their product to be...
13 February 2018 5,018 4 View
Just curious if there can be somaclonal variation in direct organogenesis or not; because in direct organogenesis i.e. from explant to shoot and then root occurs directly without the intermediate...
06 August 2017 2,074 4 View
I talked with one of my professor; he told that chimeric plants are mostly found in T0 generation only and the opportunity for finding a chimeric T1 generation plant is extremely rare or most of...
13 June 2017 4,135 25 View
I am doing a 12% SDS PAGE gel after total plant protein extraction from cotton leaf by TCA/ACETONE precipitation method. I am loading the crude protein without dilution after keeping it for...
28 April 2017 815 21 View
I want a total plant protein extraction protocol specific for cotton leaf to do SDS-PAGE of the extracted total plant protein. what should be the pH of the extraction buffer? I want the proteins...
27 February 2017 7,729 3 View
What I have heard is the normal Taq Polymerase which we commonly use can amplify a fragment upto 1.5 kb. But yesterday someone told me that the normal Taq Pol can amplify a fragment upto 3kb...
23 September 2016 1,883 3 View
This method of PCR amplification produces large concatamers of multi-looped amplicons those may show prominent visibility on the gel. But how this confers specificity of the amplification. Or if...
13 April 2016 2,481 3 View
Is it possible that combs have accumulated DNA samples from previous loadings. Because for me it seems that every time we run a gel we first remove the comb just after casting and then only run...
10 April 2016 2,345 8 View
After removing the gel solution from the microwave we keep it for cooling for nearly 10 to 15 minutes so that the temperature reduces to 55-60 degrees and then only add EtBr. Why can't we directly...
13 March 2016 5,060 3 View
