Vineeth A Raveendran

4 Questions 3 Answers 0 Followers

Questions related from Vineeth A Raveendran

Can I use a luciferase shRNA or any scrambled shRNA as a negative control for shRNA targeting my gene of interest?

Hi All, This seems to be a fairly common yet widely ignored question to me. Ideally a scrambled shRNA should have the same sequence but in random order, but how widely is it endorsed and how...

29 July 2020 3,064 0 View

Using pCMV-eGFP as an empty control for pCMV-ProteinX-GFP?

I have a really old construct that contains GFP (original) tagged protein under CMV promoter. I can't find an empty vector containing GFP (Addgene or similar). The closest they have is eGFP under...

11 March 2019 7,081 3 View

Quantification of mitochondrial elongation in Image J ?

Hello,I am trying to quantify the length of elongated mitochondria using ImageJ. I have been using Ferret diameter, but I guess this gives me an underestimation of actual length. Is there any way...

11 July 2016 5,530 3 View

Any advice in homology modelling of a protein with templates only showing an identity of ~40% with certain(imp)regions of my model seq?

using online tools(I-TASSER et al) and the results are not quite as expected.. shall I go forward with the model or is there any way to rectify this problem. Thanks in advance,Happy new year

02 January 2016 1,830 9 View