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02 August 2020 7,331 3 View

I have created a survey for my research about leadership and change. https://www.surveymonkey.com/r/CB72MGCan you please help me by taking the survey?

https://www.surveymonkey.com/r/CB72MG6

08 February 2020 4,048 1 View

Whats the optimum dose of TNFa neutralizing Ab for an invivo study?

Has anyone used any of the mouse TNFa neutralizing antibody in vivo? Whats the ideal dose that i could possibly use?

09 September 2019 5,765 1 View

Using pCMV-eGFP as an empty control for pCMV-ProteinX-GFP?

I have a really old construct that contains GFP (original) tagged protein under CMV promoter. I can't find an empty vector containing GFP (Addgene or similar). The closest they have is eGFP under...

10 March 2019 6,929 3 View

How to measure the quality matrices of a journal?

Many researchers and scientists are in confusion about judging the quality of a journal. Mere impact factor cannot measure all performance indicators of a journal. The following 5 indicators...

29 December 2017 9,392 9 View

Any one have or know about the availability of destabilized Green Fluorescent protein expressing cell line ??

Hi I m looking for destabilized GFP (deGFP) expressing cell line or vectors (viral vectors to make stable expressing cell line). I need this cell line to study the release kinetics of...

30 October 2017 225 3 View

How to find CNV from RADseq data?

How to find Copy Number Variations from RAD/ddRAD sequencing data. Is there any software available for the same

29 August 2017 5,315 3 View

Can anybody suggest me a list of wireless card drivers that support OCB(Outside the context of a basic station set) mode ?

I have tried out the ath9k driver with an appropriate atheros card. But while setting the card to ocb mode I recieved an error.These are the commands I used. sudo iw reg set DEsudo ip link set...

17 October 2016 7,219 3 View

RNA with low 260/230 =<1, showing two peaks, one close to 230 and 270?

Datasheet (Nanodrop 2000) and others said possibly these peaks are due to phenol contamination. Methods like 1- Butonol/ sodium acetate re-precipitations have shown to improved the purity of RNA....

25 July 2016 8,903 14 View

Quantification of mitochondrial elongation in Image J ?

Hello,I am trying to quantify the length of elongated mitochondria using ImageJ. I have been using Ferret diameter, but I guess this gives me an underestimation of actual length. Is there any way...

10 July 2016 5,398 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View