No results; that mean no signals and curves

Maybe you have to chose the suitable and valid qPCR polymers, runing the experiment using a small samples for testing the experiment condition, and first of all you must run the reaction using the convenient PCR with the same condition (primers ,polymerase and annealing temperature), finally I recommend you for using PCR two-steps, with high annealing temperature primer up to 62 degree.

Need more information:-

1) what type of assay? Taqman? syber green?

2) do you have controls? if yes, no results for that too?

I used Taqman mastermix from ABI. I have NTC control (notemplate control) on plate in triplicates.

I ran qPCR for my target gene as well GAPDH. I found signal and melting curve for one sample with GAPDH but others no. As i said earlier, i ran all qPCR products on agarose gel and found strong intensity amplified fragments at the correct position.

You find melting curve in PCR's performed with SYBR green, taqman system does not produce melting curves. Even when it looks trivial maybe you should check that you configured the machine at the right settings of detection. If you do Taqman but configure the machine to read SYBR green you will not find anything even when you have a right amplification...

Hi Summer,

I agree with Roberto. Kindly check the settings of the detection.