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HI, i extracted the SIV RNA from 400 ul plasma and eluted in 30 ul of RNAse/DNAse free water. I run qPCR assay to determine the viral copy number. i used 4ul of RNA in my qPCR reaction. i used...
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I ran a qPCR reaction and found no results but ran the qPCR products on gel and found amplified atrget fragments at specific positions
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i was extracted theRNA form PBMCS from macaque, they were infected with SIVmac251 and put on ART therapy. i was bale to amplify actual long size band around 2920bp (env) in some samples but in...
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HI Everyone, i am doing Quantitative viral outgrowth assay (Q - VOA). I have isolated the CD4T cells form PBMCs of SIV infected Rhesus Macaque. I did the 5 fold serial dilution, starting from...
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