19 September 2017 4 504 Report

We have synthesized some inhibitors for PKM2 enzyme. we are planning to perform enzyme kinetic studies. we studied some protocols for the same but conc. of enzymes to be used are missing. some of them suggest varrying enzyme conc while using the same conc. of substrate, while others mention varying substrate conc. Please suggest how to start. 

if someone has protocol for same please share.

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