Recommendations on CO2 Resistant Rocking Shaker?

A certain protocol we have developed requires the use of a Rocker/Rocking Shaker in which our dishes with cells are maintained in the incubator. The rocking is essential to the protocol but we...

09 February 2021 3,291 1 View

Do you consider it is important to vindicate Spanish as language, in scientific issues and publication of scientific articles?

03 February 2021 3,756 4 View

How to calculate the difference in the electron density of a dimer and its monomers?

Hello, I need to calculate the difference of the electron density of a dimer and the the sum of its monomers. I tried G09 without success, even using nosymm in all calculations. The problem...

15 January 2021 9,537 3 View

Could anyone tell me any molecular biology company that sequences mitochondrial genome?

I'm looking for a company to sequence the complete mitochondrial genome of some species of fish.

14 December 2020 3,154 3 View

How to know if 87Sr/86Sr is reliable for Sr isotope chemostratigraphy in marbles?

I am currently looking for parameters to know if 87Sr/86Sr data is reliable for chemostratigrahy in marbles, and/or parameters to know if those marbles have had some post-depositional...

30 November 2020 9,032 8 View

How to interpret hierarchical regression when a predictor is no longer significant after new variables are added?

Hello everyone: What does it mean when a variable is significant in one step but then in the next step it's no longer significant? For example, in this article...

29 November 2020 7,354 3 View

How to estimate the optimal cutpoint for a binary outcome in python?

Hello everyone, I have a dataset of diabetic patients which has been used to train an xgboost model in several outcomes such as stroke, amputation, and more. Originally we used the continuous...

21 September 2020 4,970 3 View

Does anyone has achieved the installation of gromacs plugin in PyMol, complete in Linux, with its graphic interface?

I am using Ubuntu 20.04 and I have PyMol installed, with a license. But it has been impossible to add the repository ppa:makson96/dynamics. Even downloaded from github, the package with the...

20 September 2020 6,870 1 View

How to perform power analysis for a one-way ANOVA in R when treatments variances are not exactly the same?

Hi! I've been trying to perform a power analysis for a one-way ANOVA in R using the pwr package. The equation that is employed for this is based on Cohen's f and assumes that variances among the...

16 September 2020 6,098 3 View

How do you find the electrons that occupy a particular orbital in VASP?

I think this question has already been presented here on this forum, but I was not very clear. I'm running VASP calculations involving a mixture of transition metal atoms like Fe, Mn and Co in...

07 September 2020 7,228 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

RNA extraction from exocrine pancreas?

Hi everyone ! Does anyone have any experience in extracting RNA from exocrine pancreas (which is very high in RNAse)? The pancreas is frozen tissue. I wonder if RNAse is also high in the nucleus,...

31 January 2021 390 2 View

How to preserve wet fungus spore ?

I need to preserve broth culture of wet fungus spore for my further research. Can any one suggest me any solution.

18 January 2021 4,756 2 View

Does long term preservation (10years) of Collembola in 99% Ethanol affect recovery rate of extraction by flotation?

Hello! I am currently developing an automated counting approach using Fiji to count Collembola from pitfall trapped samples that have been stored in Ethanol (99%) since 2010. I use flotation (tap...

13 January 2021 659 2 View

Matlab: train random forest with time series? How to transform time series data to a supervised learning dataset?

Hey, I've build random forest using Matlab Machine-Learning Toolbox Function (treeBagger). I've computed several kinematic features like velocity or acceleration as predictors (24 predictors) for...

22 December 2020 2,638 3 View

IHC-P staining of the viral infection in the mouse pancreas?

Hi, I'm trying to stain a viral infection of the pancreas in paraffin sections of adult (3 month old) BALB/c mice. Prior to starting my histology analysis, I have titrated the organ to confirm...

10 December 2020 2,198 3 View

Why some basidiomycete cultures died quickly when stored at 5C on plate or slopes?

Why some basidiomycete cultures died quickly when stored at 5C on plate or slopes? and is there any method to return them back to life ? specially if the culture produce anamorphic stage ? and...

09 December 2020 4,631 3 View

Buffy Coat preparation? What freezing media is best for ensuring optimal integrity?

Hello all. I am trying to isolate buffy coat from whole blood (sodium citrate), and planning on cyropreserving it for later analysis (~3-5 years). As to what analysis would include, we are leaving...

17 November 2020 1,899 3 View

Why do researchers use amylase and lipase from porcine pancreas?

I found that many researchers use amylase and lipase from the porcine pancreas to research the inhibitory activity of those natural extracts. Why they choose amylase and lipase from the porcine...

17 November 2020 4,672 8 View