Good day,
Does anyone have any experience with the purification of commercial glutaraldehyde (GA) using activated charcoal?
I am currently using the method as described by:
Anderson 1967. PURIFICATION AND QUANTITATION OF GLUTARALDEHYDE AND ITS
EFFECT ON SEVERAL ENZYME ACTIVITIES IN SKELETAL MUSCLE. The Journal of Histochemistry and Cytochemistry.
Accordingly, commercial GA is mixed with 20 % (w/v) activated charcoal and then vacuum filtered. The filtrate is again mixed with 20 % (w/v) charcoal and filtered. The process is repeated four times in total. The absorbance of the end product (final filtrate) is measured at absorbance 280 nm and 235 nm. The acceptance criteria for having purified (monomeric GA) is that the ratio of absorbance 280/235nm should be >1.5.
The method does not seem to work. I am using alkaline steam activated charcoal with a high surface area. Am I missing something? Any assistance would be much appreciated.
Dear Angélique Lewies , besides the fact that in the 1980ies I did such "purfication >#* purification #*#*
Dear Angélique Lewies , besides the fact that in the 1980ies I did such "purfication >#* purification #*#*
Dear Wolfgang H. Muss
Thank you very much for your comment and the information. For our application we need large quantities of the purfied GA-solution, therefore we opted for the purification of GA solution (50 wt % in H2O, Sigma) to try to make it more cost effective... It is nice hearing from someone who has extensive experience with this method.
However, taking into account the frustation with the method (as you also have experienced) we are considering ordering "purified GA -solution" instead.
Best wishes.
Dear Angélique Lewies , thank you for replying with some detail of your application. And thank you also for your kind assessment of the situation. Perhaps of interest (as out of my memory): most frustrating too was the fact, that the original volume of the impure G— solution by the filtering through activated charcoal was reduced to a really small volume (approx. only 1/3 could be recovered....)
Disclaimer:
Only as a satisfied (former) consumer/client, I always was happy with the quality as well as the delivery service of polysciences, Ltd.
best regards & wishes,
WHM (via iPhone)
Correction added 2020-10-15_17:15 (CEST): the mentioned Chem-Company [and Supplier of goods used in LM & EM deserves an 'Inc.' rather than an 'Ltd'-assignment (;-||)
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