During isolation of endolichenic fungi (EF), how can we ensure that EF were isolated and not the mycobiont? Is there a chance that the mycobiont be isolated rather than EF?
did you use a selective medium ?
logically, we have to separate the targeted mycobiont from its host (fungus tallus)...but, us for endophytic fungi, the host shoud be surface-sterilized..
Since the thallus is segmented, you must observe the emergence of mycobionte hyphae at the point of segmentation (with magnification tool) from the first days..or at least early....
Thank you for your answer. I used Malt Yeast Extract Agar, since this is the commonly used agar for the isolation of endolichenic fungi. But I also read that this medium can also be used for the isolation of the mycobiont.
So meaning, it will only be based on microscopy? I am working on this for my dissertation but I am not sure how to justify that what I have isolated are really EF. I did surface-sterilisation so I can be sure that I won't isolate the epiphytes.
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