I'm trying to do some proof of concept aptamer generation for proteins (like thrombin or insulin, things with published aptamer sequences to comparison). I'm trying to use the most standard techniques, nothing fancy. I've found plenty of detailed cell SELEX protocols out there, but not too many for basic protein aptamer generation. Any advice, specifically materials for protein immobilization (protein A beads? columns? etc.) would be appreciated!
We were looking to just do some proof-of-concept experiments for a protein like thrombin that has a well established sequence. We're eventually looking to move into cell SELEX but need to make sure we have everything optimized for the process with a known result.
I did find some review papers on some of these methods. Thanks for the summary, Jon!
I was really wondering if anyone had a cohesive protocol for protein aptamer generation (similar to the Nature protocol for cell SELEX).
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