Protocol to study Ascorbic acid in vegetable sample through HPLC?

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Kou Hayakawa

I would like to recommend HPLC-photometric method of Assoc.Prof. Ing.Věra Schulzová, Ph.D. (Praha, Czech).

Aly R Abdel-Moemin

Dear Muhammad Irshad,

The method suggested by Kou is good also try to read the attached. We have a developed method that is highly recommend for you in the link below.

http://www.sciencedirect.com/science/article/pii/002196739280329S

Hope this help

Gordon Gribble

The J.Chem.Ed. over the past several years has a procedure. Good luck.

O. V. Dolotova

Rather good method for HPLC analysis of Ascorbic acid is following:

Column X-Bridge Waters C 18, 150x4,6 mm (3,5 μm). T=25 ºС. Total Flow 0,8 mL/min. UV detection at 210 nm, t=12 min. Isocratic gradient: A=15,6g NaH2 PO4 •2H2O in 1L of water (рН=2,5).

With best wishes,

Olga Dolotova