I am not an expert in this field, but I am very interested and have researched to find an answer. I received some assistance from tlooto.com for this response. Could you please review the response below to see if it is correct?

The SP2/O toxicity assay assesses the cytotoxic effects of substances on SP2/O myeloma cells, commonly used in evaluating tissue culture media. Although specific protocols are not easily found in literature, adapting general cytotoxicity assays like MTT or XTT is feasible. SP2/O cells are cultured in test media, exposed to the substance, and cell viability is measured over time. Viability is determined through metabolic activity indicators or cell counting. Optimizing variables such as cell density, exposure time, and substance concentration is essential for accurate assessment [1][2]. This approach allows the assay to reflect cytotoxic impacts effectively.

Reference

[1]

Ghasemi, M., Turnbull, T., Sebastian, S., & Kempson, I. (2021). The MTT Assay: Utility, Limitations, Pitfalls, and Interpretation in Bulk and Single-Cell Analysis. International Journal of Molecular Sciences, 22.

[2]

Judson, R., Houck, K., Martin, M. T., Richard, A., Knudsen, T., Shah, I., Little, S., Wambaugh, J., Setzer, R., Kothiya, P., Phuong, J., Filer, D., Smith, D., Reif, D. M., Rotroff, D. M., Kleinstreuer, N., Sipes, N. S., Xia, M., Huang, R., ... & Thomas, R. S. (2016). Analysis of the Effects of Cell Stress and Cytotoxicity onIn Vitro Assay Activity Across a Diverse Chemical and Assay Space. Toxicological Sciences, 153, 409 - 409.

Park Sowon I really appreciate your assistance on this, we had been speculating the same method as a means of analyzing the medium's suitability. This does help us.