Can anyone help me by providing a detailed protocol for western blot in drosophila model of AD?
I am still waiting for reply
I.e. what is the protein extraction process from drosophila head with protein extract buffer composition along with step wise protocol for gel running condition upto transfer?
04 May 2014 2,613 1 View
I want to run the 18% gel so please tell me what should be the condition for that staring from running voltage upto transfer on membrane. For isolation of protein from drosophila head for western...
05 June 2013 4,403 4 View
I would like to separate and identify the whole polyphenolic component present in an herbal extract. So can anybody please help me by providing full details or respective protocols which I should...
11 December 2012 5,303 43 View
I am working on AD using drosophila model system. I want to go for the western blotting from the head region of drosophila, So can anyone help me by providing the following details- 1. what should...
09 October 2012 3,561 8 View
Can anyone please suggest what Ishould do for the isolation of different components from the crude herbal extract?
03 April 2012 1,769 12 View
If i want to clone our desire gene with GFP in pUAST vector, then can anyone please tell me that how I should proceed?........
09 October 2011 2,792 6 View
I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...
25 February 2021 8,404 3 View
I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...
24 February 2021 8,514 3 View
Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...
23 February 2021 8,609 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...
22 February 2021 9,901 3 View
I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...
22 February 2021 6,937 3 View
I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...
22 February 2021 1,777 3 View
Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...
17 February 2021 4,844 4 View
Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...
17 February 2021 1,130 6 View
I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...
12 February 2021 5,824 3 View