Can anyone help me by providing a detailed protocol for western blot in drosophila model of AD?
I am still waiting for reply
I.e. what is the protein extraction process from drosophila head with protein extract buffer composition along with step wise protocol for gel running condition upto transfer?
04 May 2014 2,696 1 View
I want to run the 18% gel so please tell me what should be the condition for that staring from running voltage upto transfer on membrane. For isolation of protein from drosophila head for western...
05 June 2013 4,499 4 View
I would like to separate and identify the whole polyphenolic component present in an herbal extract. So can anybody please help me by providing full details or respective protocols which I should...
11 December 2012 5,389 43 View
I am working on AD using drosophila model system. I want to go for the western blotting from the head region of drosophila, So can anyone help me by providing the following details- 1. what should...
09 October 2012 3,722 8 View
Can anyone please suggest what Ishould do for the isolation of different components from the crude herbal extract?
03 April 2012 1,851 12 View
If i want to clone our desire gene with GFP in pUAST vector, then can anyone please tell me that how I should proceed?........
09 October 2011 2,885 6 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
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Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?
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I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
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I know mass spectrometry will work. But, is there any simple and straightforward method to distinguish between the two proteins of the same molecular weight? They are cryptochromes and...
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Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
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Hello, I isolated total histones successfully from the skin primary cells by acid extraction. I wanted to detect histone H1.2 expression in different conditions by western blot. Can some one...
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Hello! I am planning a western blot experiment, and I am looking for GAPDH antibodies to order. In the past, I have experienced a lot of hit-or-miss when ordering antibodies based on just the...
07 July 2024 1,685 0 View
I have this problem with my western blot for the ABCG2 antibody on nitrocellulose membrane. Help please
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I have used this BIorad Powerpac Basic for 4 years now, before which it was used for another 5-6 years. Generally we addressed the E1 error by compensating with fresh buffer and/or filling the...
01 July 2024 7,104 1 View