Sandeep Singh

7 Questions 31 Answers 0 Followers

Questions related from Sandeep Singh

Can anyone please help me out for providing the exact protocol for the western blot analysis for Abeta from drosophila head?

I.e. what is the protein extraction process from drosophila head with protein extract buffer composition along with step wise protocol for gel running condition upto transfer?

05 May 2014 2,673 1 View

Regarding western for amyloid beta from drosophila head

I want to run the 18% gel so please tell me what should be the condition for that staring from running voltage upto transfer on membrane. For isolation of protein from drosophila head for western...

06 June 2013 4,476 4 View

I would like to identify and quantify all the polyphenolic component in a herbal extract, so can anyone please help me regarding this problem?

I would like to separate and identify the whole polyphenolic component present in an herbal extract. So can anybody please help me by providing full details or respective protocols which I should...

12 December 2012 5,365 43 View

Regarding Protein Isolation from Head region of drosophila for western blotting

I am working on AD using drosophila model system. I want to go for the western blotting from the head region of drosophila, So can anyone help me by providing the following details- 1. what should...

10 October 2012 3,688 8 View

Protocol for Western Blot in drosophila AD model

Can anyone help me by providing a detailed protocol for western blot in drosophila model of AD?

05 May 2012 3,048 1 View

Methods for the Isolation of different components from crude extract of a plant, specially poly-phenolic and flavonoid content

Can anyone please suggest what Ishould do for the isolation of different components from the crude herbal extract?

04 April 2012 1,828 12 View

what should be the position of GFP in vector, if i want to clone our desire fragment with GFP

If i want to clone our desire gene with GFP in pUAST vector, then can anyone please tell me that how I should proceed?........

10 October 2011 2,860 6 View