I.e. what is the protein extraction process from drosophila head with protein extract buffer composition along with step wise protocol for gel running condition upto transfer?
Dear sandeep you search ALZFORUM site there u got protocol for western blotting for Abeta
I want to run the 18% gel so please tell me what should be the condition for that staring from running voltage upto transfer on membrane. For isolation of protein from drosophila head for western...
05 June 2013 4,499 4 View
I would like to separate and identify the whole polyphenolic component present in an herbal extract. So can anybody please help me by providing full details or respective protocols which I should...
11 December 2012 5,389 43 View
I am working on AD using drosophila model system. I want to go for the western blotting from the head region of drosophila, So can anyone help me by providing the following details- 1. what should...
09 October 2012 3,722 8 View
Can anyone help me by providing a detailed protocol for western blot in drosophila model of AD?
04 May 2012 3,067 1 View
Can anyone please suggest what Ishould do for the isolation of different components from the crude herbal extract?
03 April 2012 1,851 12 View
If i want to clone our desire gene with GFP in pUAST vector, then can anyone please tell me that how I should proceed?........
09 October 2011 2,885 6 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...
06 August 2024 8,981 8 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have a theoretical framework for preparing a interview guide to assess the unmet care needs of a sub population of chronically ill patients and their caregivers(dyadic interviews).
04 August 2024 8,798 7 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View