Can anyone please help me out for providing the exact protocol for the western blot analysis for Abeta from drosophila head?

I.e. what is the protein extraction process from drosophila head with protein extract buffer composition along with step wise protocol for gel running condition upto transfer?

04 May 2014 2,613 1 View

Regarding western for amyloid beta from drosophila head

I want to run the 18% gel so please tell me what should be the condition for that staring from running voltage upto transfer on membrane. For isolation of protein from drosophila head for western...

05 June 2013 4,403 4 View

I would like to identify and quantify all the polyphenolic component in a herbal extract, so can anyone please help me regarding this problem?

I would like to separate and identify the whole polyphenolic component present in an herbal extract. So can anybody please help me by providing full details or respective protocols which I should...

11 December 2012 5,303 43 View

Regarding Protein Isolation from Head region of drosophila for western blotting

I am working on AD using drosophila model system. I want to go for the western blotting from the head region of drosophila, So can anyone help me by providing the following details- 1. what should...

09 October 2012 3,561 8 View

Protocol for Western Blot in drosophila AD model

Can anyone help me by providing a detailed protocol for western blot in drosophila model of AD?

04 May 2012 2,977 1 View

what should be the position of GFP in vector, if i want to clone our desire fragment with GFP

If i want to clone our desire gene with GFP in pUAST vector, then can anyone please tell me that how I should proceed?........

09 October 2011 2,792 6 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View