Protocol for the virus production in HeLa cells?

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I want to do research on business related issue.Do you have any idea what topic i should choose?

its about my masters dissertation

10 June 2024 5,490 7 View

I want determine Iron by spectrophotometer? Anyone know about the spectrophotometric determination of iron and copper, kindly share the procedure?

spectrophotometric determination of iron and copper

04 June 2024 1,720 0 View

How we can find thermoelectric properties of 2D by VASP?

Hello Seniors, I need some help, I want to find out thermoelectric properties of 2D materials by VASP software can you tell me All steps and important INCAR tags.?

02 June 2024 7,093 1 View

Since many centuries human has researched on advancing weapons of destruction, why not researched on ways to bring advancement for peace in the world?

The mankind is on the edge of resistance to peace. If you view news you will find that every day there are portions of the world where wars are happening and many innocent people are being killed....

26 May 2024 4,633 3 View

How to increase yield of silver nanoparticles from methanolic nigella sativa seed extract?

I have used 50 ml 1 mM silver nitrate solution and 5 ml extract and 30 microliters of base

15 April 2024 7,326 1 View

What is the difference between Calibrator. Control and Standard?

i am working in a diagnostic lab and very confusing about the calibrator, control and standard that we are using in our lab for quality assuarance of diagnosis of disease

07 April 2024 5,763 2 View

How to calculate the crystallization temperature of the Illite/Smectite (mixed clay sediments) from stable hydrogen and oxygen isotopic values?

I want to find out the paleoenvironmental changes across the different sections (P-T transition sediments) of my study area (Southwestern China), based on the measured oxygen and hydrogen isotope...

05 March 2024 4,298 0 View

Devise a cloning strategy to clone Chitinase gene from Spodoptera frugiperda into maize against a fungal pathogen Alternaria alternata?

If I have to make the cloning strategy, what should be my workflow to do this. I got two problems, one to select suitable expression vector from pCAMBIA1301, and pCAMBIA1302. As 1301 has GUS...

17 February 2024 8,748 2 View

About gene search in NCBI?

If the gene sequence for a specific gene (e.g., Asparaginase) is not found in the NCBI database, how can explore alternative strategies to identify and obtain the gene sequence in the organism of...

02 February 2024 1,910 3 View

What are some effective ways to deal with the premature convergence of the Particle Swarm Optimization (PSO)?

I am working on a project that implements PSO to solve a nonlinear optimization equation (minimization problem). I want to ensure that PSO is not falling for premature convergence (i.e choosing a...

16 January 2024 6,905 3 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How can I improve the quality of lamb milk replacer for machine feeding?

Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...

06 August 2024 6,227 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

05 August 2024 2,977 3 View

Ali Ahmed Azzawri

https://www.hollingscancercenter.org/research/shared-resources/shRNA/Lentivirusproduction-shRNA.pdf

https://www.addgene.org/protocols/lentivirus-production/

Jatin Chadha

Hello Naveed Shahzad

Can you please suggest the virus you would want to propagate if using for any gene silencing or related purposes, what sort of plasmid you are making use of? It would be better to supplement this information.

Naveed Shahzad

@Jatin: It's a BLV provirus cloned in a psp64 addgene vector.