Can anyone please share the protocol of live virus production in Hela cells by using infectious viral plasmid?
https://www.hollingscancercenter.org/research/shared-resources/shRNA/Lentivirusproduction-shRNA.pdf
https://www.addgene.org/protocols/lentivirus-production/
Hello Naveed Shahzad
Can you please suggest the virus you would want to propagate if using for any gene silencing or related purposes, what sort of plasmid you are making use of? It would be better to supplement this information.
@Jatin: It's a BLV provirus cloned in a psp64 addgene vector.
its about my masters dissertation
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