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Can anyone please share the protocol of live virus production in Hela cells by using infectious viral plasmid?
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I am trying to transfect Hela cells with a large 12 kb plasmid by using Lipofectamine 2000. I have used various concentrations (1,2,3,4 and 5 ug) of the plasmid and transfection reagents but the...
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Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
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