Protocol for supernatant secretory protein in min6 cells?

06 October 2023 1 10K Report

I want to identify a secretory protein that is secreted from min6 cell in the supernatant can anyone provide protocols?

Firs I want to get conditioned media and then precipitate after that I will do SDS-PAGE and CBB/silver staining. I am a newbie so I don't know how much medium, how much cells is needed for this assay, I couldn't find a good reference

Malcolm Nobre

Hello Sya Retno,

I have found a good reference for you. The work done is almost similar to the work you plan to carry out for your project.

Please refer to the paper attached below. The protocol provided in the article will be helpful.

https://doi.org/10.1038/s41598-017-04456-4

Best.

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