does serum starvation need to be done in the exponential growth cells? or is it okay to do it 20hr after seeding? my cells is a slow growth type cells that has ~48 hours doubling time
I did an experiment for a positive control phos-tag system to detect phosphorylated AMPK in min6 cells. I incubated min6 cells for 12h with metformin to induce AMPK phosphorylation then check the...
06 January 2024 7,752 1 View
can a gene cause slow growth phenotype in vitro but also in qPCR has down regulation on p16?
05 November 2023 1,121 0 View
I want to identify a secretory protein that is secreted from min6 cell in the supernatant can anyone provide protocols? Firs I want to get conditioned media and then precipitate after that I will...
05 October 2023 9,963 1 View
I did cell synchronization by serum starvation to my cultured mammalian cells for 24 hours and I did FACS analysis, however I found no G1 arrest, I prolong the starvation for 72 hours but I got...
07 July 2023 2,915 1 View
Is it possible that a KD cells showed reduced cell proliferation by WST but during FACS analysis it did not show any change of cell cycle? apoptosis checking by cleaved caspase 3 western blot also...
26 June 2023 3,670 1 View
I am a newbie in FACS analysis: so I used this reagent from dojindo called cell cycle blue reagent and do staining in live cells (not like PI) they stated that fixation is not needeed, the problem...
08 June 2023 6,644 6 View
I am now studying a protein, this protein might affects cell proliferation because I performed a Knock down experiment and I found that the cells proliferation rate is reduced when this protein is...
08 June 2023 1,586 2 View
By retroviral over-expression system I couldn't get the same amount of over-expression efficiency (increased expression in protein level as detected by western blot). I tried overexpressing a...
28 May 2023 7,350 3 View
I performed PCR for genotyping but I could not amplify one of the sample while other samples is amplifying just fine... even I put + and - control, the + ctrl is amplifying well... what could be...
14 May 2023 3,111 8 View
Hi all, Can I use the same cells again after I put them in 96-well plates, tested them with a cell counting kit at OD450 (CCK8) at 24 hours, washed them with PBS, added new media, and let them sit...
23 April 2023 3,731 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!
08 August 2024 6,242 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
The stability of the Solar System is a complex subject that blends the classical framework of Newtonian mechanics with the modern insights provided by General Relativity (GR). Understanding this...
07 August 2024 2,569 1 View
Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...
07 August 2024 7,013 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View