Protocol for preparing western blot sample from rat pituitary gland?

Morteta H AL-Medhtiy @Morteta-Al-Medhtiy

13 March 2019 0 4K Report

Hi dear researchers,

I am going to receive rats-pituitary gland and I am going to homogenise it. I am looking to get a more details protocol for preparing lysate and detect SIRT1 by using western blot.

I have checked many different protocols but they are so superficial details. I need to know how much RIPA buffer I need to add if each pituitary 3mg?

I will be grateful if any body has good experiance to advice me with the steps?

Regards

Morteta

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