Hello!
Has anyone used MitoSOX™ Red mitochondrial superoxide indicator *for live-cell imaging* (M36008) for suspension cells? I want to analyze fluorescence with a plate reader. I am wondering whether washing step in the protocol wont affect my cells - for adherent cells is not a problem but suspension cells must be centrifuged and I don't know how to make it without affecting cells. Does anyone has experience with this analysis with suspension cells? I will be grateful for some tips!
Strictly speaking, you don't actually need to wash MitoSOX off of cells. It is virtually non-fluorescent in its non-oxidized state and should give you minimal background. If you're super concerned, simply have a no-cell well for background control.
Hi Agata,
I was wondering if you got/have a MitoSOX Red protocol for suspension cells.
Thank you.
KJ
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