Protocol for inner and outer membrane isolation from Bacillus subtilis

07 July 2014 1 7K Report

I need to isolate the inner and outer membrane proteins of Bacillus subtilis. Can someone suggest a protocol to for the same. Please suggest some papers too.

David Oppenheimer

Bacillus subtilis is a Gram-positive bacterium and does not have an outer membrane. I do not have a protocol for you, but here is a review paper that may point you in the right direction.

Zweers, J.C., I. Barak, D. Becher, A.J. Driessen, M. Hecker, V.P. Kontinen, M.J. Saller, L. Vavrova and J.M. van Dijl. 2008. Towards the development of Bacillus subtilis as a cell factory for membrane proteins and protein complexes. Microb Cell Fact 7: 10.

Badges
Science method

More Ria Biswas's questions See All

Problem with H-bond analysis in GROMACS ?

I have simulated a protein-protein complex in GROMACS. During Hydrogen bond analysis for individual residues using gmx hbond, I am getting results as H-bond average 1.012 for 2134 frames. What to...

11 December 2016 6,634 5 View

How to model a nanoparticle computationally ?

I want to study interaction studies of nanoparticle with my protein of interest. can anyone suggest me how to model a perfect nanoparticle for computational studies? what shall be the parameters...

06 July 2015 6,432 2 View

How can I dock multiple ligands to each pocket of each monomer of a homotetrameric protein?

I am trying to dock ligand to a homotetrameric protein which is functional only in its tetrameric form. My problem is I have to dock four ligands in the four catalytic pockets of each monomer....

01 February 2015 2,654 5 View

What are the best methods for removing contaminating gDNA from my RNA preparations which I will be using for RT-PCR?

See above.

05 June 2014 4,118 20 View

Does anyone know a command for separating pdb files after a simulation run in gromacs?

Can anyone tell me the command for separating pdb files after a simulation is complete in gromacs?

04 May 2014 5,884 3 View

I am unable to see any band for my PCR?

The annealing temperature of the primer is very low (35 degrees centigrade). What should be the measures? Should I redesign my primers?

11 December 2013 4,207 11 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View