Protocol for Detection of Reactive oxygen species (ROS) using CM-H2DCFDA in THP-1 cells?

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James David Londino

https://www.researchgate.net/post/What_is_the_best_or_suitable_protocol_to_detect_the_accumulation_of_reactive_oxygen_species_in_cell_culture

https://www.researchgate.net/post/DCF_DA_fluorescense-ROS_detection_in_attached_cells

Goodwin G. Jinesh

Add DCFDA 30 minutes before and incubate at 37 with CO2 and wash the cells with PBS (to reduce aggregation: or use filtering) and immediately analyze by FACS at FITC/FL-1 channel

https://www.researchgate.net/publication/299607409_Novel_PKC-z_to_p47phox_interaction_is_necessary_for_transformation_from_blebbishields

Article Novel PKC-? to p47phox interaction is necessary for transfor...

Nimra Siddiqui

Hello Prahlad Pokharel

Did you get the answer of your question? I am stuck in this step and i don't know how to do it with THP-1. When i treat the labelled THP-1 cells with H202 (Hyedrogen peroxide) . and then see the results, no ros is produced. Can you please help me. I will be very thankful to you.