We are thinking a way to stress the auditory function of mice. We are discussing a daily stimulation with sounds. But we don't yet know about amplitudes and frecuencies to use, how much and how to use them. Would you point to some bibliography?
Have to admit that I tried this once, the only one who became seriously stressed was the researcher.
I want to study the cochlear nucleus using IF. The cochlear nucleus It's a rather superficial nucleus, located on the brainstem below the most dorsal sulci of the cerebellum and lateral to where...
07 August 2018 6,714 4 View
I haven't found a complete sequence of this mutated gene, there are some older publications with figures containing pieces of the viral insertion this gene has, but I would want to know if there's...
02 March 2018 7,998 2 View
I'm looking for H&E stained slides of retina to find how does the retina look at different stages of retinal ganglion cell death in diseases like glaucoma. Haven't found much on google.
07 August 2017 632 1 View
I'm doing mutagenesis on a plasmid with mutagenic primers by lifting two overlapping semgents carrying a single point mutation (this is not the stratagene method). I will use these two sequences...
01 January 1970 5,707 15 View
So for the last couple of months I've been trying to produce cDNA from mouse tissue. My goal was to get a positive control cDNA that I was going to get from extracting and reverse transcribing RNA...
01 January 1970 6,012 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
What is the role of oxidative stress in toxin-induced carcinogenesis? I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from...
02 August 2024 3,174 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View