08 August 2017 1 633 Report

I'm looking for H&E stained slides of retina to find how does the retina look at different stages of retinal ganglion cell death in diseases like glaucoma. Haven't found much on google.

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Would superficial nuclei of the mouse brain be well fixed if instead of perfusing I sink it in PFA 4%?

I want to study the cochlear nucleus using IF. The cochlear nucleus It's a rather superficial nucleus, located on the brainstem below the most dorsal sulci of the cerebellum and lateral to where...

07 August 2018 6,714 4 View

Where can I find sequences of gene alleles and mutations? I'm looking for pde6b rd1 specifically. How could I find it?

I haven't found a complete sequence of this mutated gene, there are some older publications with figures containing pieces of the viral insertion this gene has, but I would want to know if there's...

02 March 2018 7,998 2 View

Protocol for chronic auditory stimulation/stress in mice?

We are thinking a way to stress the auditory function of mice. We are discussing a daily stimulation with sounds. But we don't yet know about amplitudes and frecuencies to use, how much and how to...

09 October 2017 8,507 1 View

PCR overlap extension with a small 29bp overlapping region (Tm=67ºC) Should I worry a 72ºC extension step?

I'm doing mutagenesis on a plasmid with mutagenic primers by lifting two overlapping semgents carrying a single point mutation (this is not the stratagene method). I will use these two sequences...

01 January 1970 5,707 15 View

Why is my RT-PCR only working for housekeeping genes?

So for the last couple of months I've been trying to produce cDNA from mouse tissue. My goal was to get a positive control cDNA that I was going to get from extracting and reverse transcribing RNA...

01 January 1970 6,012 4 View

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