Would superficial nuclei of the mouse brain be well fixed if instead of perfusing I sink it in PFA 4%?

I want to study the cochlear nucleus using IF. The cochlear nucleus It's a rather superficial nucleus, located on the brainstem below the most dorsal sulci of the cerebellum and lateral to where...

07 August 2018 6,611 4 View

Where can I find sequences of gene alleles and mutations? I'm looking for pde6b rd1 specifically. How could I find it?

I haven't found a complete sequence of this mutated gene, there are some older publications with figures containing pieces of the viral insertion this gene has, but I would want to know if there's...

02 March 2018 7,922 2 View

Protocol for chronic auditory stimulation/stress in mice?

We are thinking a way to stress the auditory function of mice. We are discussing a daily stimulation with sounds. But we don't yet know about amplitudes and frecuencies to use, how much and how to...

09 October 2017 8,423 1 View

No title

I'm doing mutagenesis on a plasmid with mutagenic primers by lifting two overlapping semgents carrying a single point mutation (this is not the stratagene method). I will use these two sequences...

01 January 1970 5,621 15 View

Why is my RT-PCR only working for housekeeping genes?

So for the last couple of months I've been trying to produce cDNA from mouse tissue. My goal was to get a positive control cDNA that I was going to get from extracting and reverse transcribing RNA...

01 January 1970 5,914 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View