Esteban Barila

6 Questions 24 Answers 0 Followers

Questions related from Esteban Barila

Would superficial nuclei of the mouse brain be well fixed if instead of perfusing I sink it in PFA 4%?

I want to study the cochlear nucleus using IF. The cochlear nucleus It's a rather superficial nucleus, located on the brainstem below the most dorsal sulci of the cerebellum and lateral to where...

08 August 2018 6,675 4 View

Where can I find sequences of gene alleles and mutations? I'm looking for pde6b rd1 specifically. How could I find it?

I haven't found a complete sequence of this mutated gene, there are some older publications with figures containing pieces of the viral insertion this gene has, but I would want to know if there's...

03 March 2018 7,969 2 View

Protocol for chronic auditory stimulation/stress in mice?

We are thinking a way to stress the auditory function of mice. We are discussing a daily stimulation with sounds. But we don't yet know about amplitudes and frecuencies to use, how much and how to...

10 October 2017 8,469 1 View

How does Retinal Ganglion cell death of glaucoma look on stained slides?

I'm looking for H&E stained slides of retina to find how does the retina look at different stages of retinal ganglion cell death in diseases like glaucoma. Haven't found much on google.

08 August 2017 594 1 View

Why is my RT-PCR only working for housekeeping genes?

So for the last couple of months I've been trying to produce cDNA from mouse tissue. My goal was to get a positive control cDNA that I was going to get from extracting and reverse transcribing RNA...

01 January 1970 5,972 4 View

PCR overlap extension with a small 29bp overlapping region (Tm=67ºC) Should I worry a 72ºC extension step?

I'm doing mutagenesis on a plasmid with mutagenic primers by lifting two overlapping semgents carrying a single point mutation (this is not the stratagene method). I will use these two sequences...

01 January 1970 5,670 15 View