A quick immunology question: Does this immunology graph seem correct?

Hi!! I have created a graph about the immune response in healthy and AD skin for S. aureus. Could I please ask if it should be a table or a figure? Does the pathway look correct? Shall I make it...

09 October 2019 7,376 1 View

50-50 dilution in elisa is better than no dilution?

Hi, I have been doing HBD-3 Elisa with highest sample concentration 50-50 dilution. I heard that 50-50 is better than adding 100uL of your sample, because you take into account the diluent as...

08 September 2018 8,773 3 View

Protocol to lyse S.aureus with Triton-X100 only?

Hi, Could you please suggest a protocol to lyse S.aureus cells with Triton-X100? I was thinking to add 10% Triton in 10^7 cell pellet and incubate for 20 minutes. What would you suggest? (I need...

08 September 2018 8,152 0 View

Sudden reduced response of HaCaT cells to stimuli - why?

Hi, I am culturing HaCaT cells, stimulating, collecting and measuring antimicrobial peptides produced. Suddenly one time the signal was relatively low (the response according to the peptide...

07 August 2018 6,129 0 View

If samples in elisa overflow can your run only these samples or do you have to run again the whole experiment with higher dilution?

If samples in elisa overflow can your run only these samples or do you have to run again the whole experiment with higher dilution?

07 August 2018 3,015 6 View

De-salting methods without losing any proteins?

Hi, I am looking for methods of removing the salt from a solution, without using buffer exchange dialysis, or spinning down using membranes, so that no proteins are lost, no matter how small they...

02 March 2018 5,811 4 View

Where can I dissolve Met-Leu and in what concentration (in order to perform MIC)?

Where can I dissolve Met-Leu and in what concentration (in order to perform MIC)? Thank you in advance. Virginia

09 October 2016 4,247 3 View

How do I dissolve D-Aspartic Acid and Ala-Phe for MICs?

09 October 2016 2,552 4 View

No title

31 December 2015 3,264 1 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View