Virginia Pappa

10 Questions 11 Answers 0 Followers

Questions related from Virginia Pappa

A quick immunology question: Does this immunology graph seem correct?

Hi!! I have created a graph about the immune response in healthy and AD skin for S. aureus. Could I please ask if it should be a table or a figure? Does the pathway look correct? Shall I make it...

10 October 2019 7,457 1 View

Protocol to lyse S.aureus with Triton-X100 only?

Hi, Could you please suggest a protocol to lyse S.aureus cells with Triton-X100? I was thinking to add 10% Triton in 10^7 cell pellet and incubate for 20 minutes. What would you suggest? (I need...

09 September 2018 8,232 0 View

50-50 dilution in elisa is better than no dilution?

Hi, I have been doing HBD-3 Elisa with highest sample concentration 50-50 dilution. I heard that 50-50 is better than adding 100uL of your sample, because you take into account the diluent as...

09 September 2018 8,837 3 View

If samples in elisa overflow can your run only these samples or do you have to run again the whole experiment with higher dilution?

08 August 2018 3,064 6 View

Sudden reduced response of HaCaT cells to stimuli - why?

Hi, I am culturing HaCaT cells, stimulating, collecting and measuring antimicrobial peptides produced. Suddenly one time the signal was relatively low (the response according to the peptide...

08 August 2018 6,186 0 View

De-salting methods without losing any proteins?

Hi, I am looking for methods of removing the salt from a solution, without using buffer exchange dialysis, or spinning down using membranes, so that no proteins are lost, no matter how small they...

03 March 2018 5,873 4 View

Where can I dissolve Met-Leu and in what concentration (in order to perform MIC)?

Where can I dissolve Met-Leu and in what concentration (in order to perform MIC)? Thank you in advance. Virginia

10 October 2016 4,306 3 View

How do I dissolve D-Aspartic Acid and Ala-Phe for MICs?

I want to perform MICs for staphylococci with D-Aspartic Acid (soluble in 1M NaOH 50mg/mL) and Ala-Phe (soluble in 80% acetic acid). However, when using these diluents it seems that it is the...

10 October 2016 2,618 4 View

Protocol for bacteria competition assays?

Hi, I would like to perform competition assays for two staphylococcal strains with and without reagents in order to see which one grows better. Do you have any protocol for this? I was thinking of...

10 October 2016 6,089 2 View

How can I dissolve Linoleic Acid, γ-Linolenic acid and Arachidonic acid and what diluent should I use for MIC determinations?

Hi, I am interested in performing MIC determination for staph aureus for Linoleic Acid γ-Linolenic acid Arachidonic acid and I would like to confirm how I need to prepare the solutions and what...

01 January 2016 3,346 1 View