A quick immunology question: Does this immunology graph seem correct?

Hi!! I have created a graph about the immune response in healthy and AD skin for S. aureus. Could I please ask if it should be a table or a figure? Does the pathway look correct? Shall I make it...

09 October 2019 7,376 1 View

50-50 dilution in elisa is better than no dilution?

Hi, I have been doing HBD-3 Elisa with highest sample concentration 50-50 dilution. I heard that 50-50 is better than adding 100uL of your sample, because you take into account the diluent as...

08 September 2018 8,773 3 View

Protocol to lyse S.aureus with Triton-X100 only?

Hi, Could you please suggest a protocol to lyse S.aureus cells with Triton-X100? I was thinking to add 10% Triton in 10^7 cell pellet and incubate for 20 minutes. What would you suggest? (I need...

08 September 2018 8,152 0 View

Sudden reduced response of HaCaT cells to stimuli - why?

Hi, I am culturing HaCaT cells, stimulating, collecting and measuring antimicrobial peptides produced. Suddenly one time the signal was relatively low (the response according to the peptide...

07 August 2018 6,129 0 View

If samples in elisa overflow can your run only these samples or do you have to run again the whole experiment with higher dilution?

If samples in elisa overflow can your run only these samples or do you have to run again the whole experiment with higher dilution?

07 August 2018 3,015 6 View

De-salting methods without losing any proteins?

Hi, I am looking for methods of removing the salt from a solution, without using buffer exchange dialysis, or spinning down using membranes, so that no proteins are lost, no matter how small they...

02 March 2018 5,811 4 View

Where can I dissolve Met-Leu and in what concentration (in order to perform MIC)?

Where can I dissolve Met-Leu and in what concentration (in order to perform MIC)? Thank you in advance. Virginia

09 October 2016 4,247 3 View

Protocol for bacteria competition assays?

Hi, I would like to perform competition assays for two staphylococcal strains with and without reagents in order to see which one grows better. Do you have any protocol for this? I was thinking of...

09 October 2016 5,978 2 View

How do I dissolve D-Aspartic Acid and Ala-Phe for MICs?

09 October 2016 2,552 4 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View