Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View

Does SSMPCA toolbox (scanvp) provide a module to perform the bootstrapping procedure to estimate the reliability of the covariance pattern?

I'm learning to perform the multivariate covariance analysis with SSMPCA toolbox (scanvp/ssmpca, www.feinsteinneuroscience.org). The step-by-step procedure to obtain covariance pattern and...

08 February 2021 2,383 1 View

Has anybody used the Chicken anti-mCherry antibody (ab205402) in immunohistochemistry?

I'm looking for a primary antibody to detect tdTomato in spinal cord slices of mutant mice. I know that the most widely used antibody for this purpose is the mouse anti-RFP from Rockland, but I...

02 February 2021 5,515 3 View

I am culturing murine bone marrow derived mesenchymal stromal cells, what are these cells I am observing?

Dear all, I am isolating mesenchymal stromal cells (MSCs) from the bone marrow of adult C57BL/6 mice. The culture conditions are: -IMDM + L-glut, 20% of 50:50 FBS:HS, 1% P/S -Medium replaced each...

24 January 2021 7,223 3 View

I am inducing ostegenic differentiation in murine bone marrow derived mesenchymal stromal cells, what are these cells I am observing?

Dear all, I am isolating mesenchymal stromal cells (MSCs) from the bone marrow of adult C57BL/6 mice. The culture conditions are: -IMDM + L-glut, 20% of 50:50 FBS:HS, 1% P/S -Medium replaced each...

24 January 2021 3,678 3 View

How can I create a custom corpus for text mining with nltk/spacy?

Hi, I am quite a newbie with python, and I need to run some text mining analysis on 100+ literary texts in German, which I have stored as individual txt files in a folder. They are with the scheme...

20 September 2020 4,762 3 View

Which rapid in-line/on-line method could be used to ensure extraction yield for instant coffe and how to calibrate it on chemometric methods?

There is a need to ensure extraction yield of the soluble coffee process, thus we need a method to analyze carbohydrate degradation products produced after hydrolisys of polysaccharides due to...

11 March 2020 3,308 2 View

How high can be the solar radiation measured outdoor in Australia?

Dear all, I am using a weather station to measure some environmental parameter in the city of Sydney, pedestrian level. In summertime, the global solar irradiation (horizontal) returned by the...

08 March 2020 7,599 6 View

Anyone having problems with low DNA concentrations after RNase A treatment (during DNA extraction)?

I am currently testing the GeneAid DNA extraction kit on abalone (Haliotis iris) attachment muscle tissue samples and it was working pretty fine, until I added the RNase A treatment (as stated in...

26 January 2020 3,019 1 View

HPLC for AAV purification?

Hello, I'm looking into buying a new HPLC machine for purification of AAV preps. Has anyone had a good (or bad) experience with a particular model? Thank you.

22 January 2020 4,024 6 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

Why is my qPCR suddently not working?

I've been working with qPCR for some months now. I amplify 2 genes (PSEN2 and HN1), and the protocols are optimized for both and have been working fine. The other day I amplified HN1 for some...

21 February 2021 3,437 3 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Agarose gel purification pre MinION sequencing?

Dear all, we performed an agarose gel purification with MinElute kit (QIAGEN) to extract bands between 3500 bp and 6000 bp for further sequencing with MinION. After the purification process,...

16 February 2021 5,178 1 View