How can we measure the reliability and validity of our survey questionnaire?

We are conducting survey research on COVID-19 misinformation among students. How can we validate our survey for it to produce valid and reliable data? Also, what statistical tools can we use to...

16 February 2021 678 3 View

How to design a reactive extraction process in Aspen Plus?

Hello, my task is it to create a specific extraction process. There is a mixture containing water, nitric acid and hydrochloric acid. It is known, that tributyl-phosphate is a solvent, which...

03 January 2021 4,163 3 View

Could speech recognition software be developed that DOESN'T convert sounds into waveforms? If not, what does it say about the semiotics of knowledge?

The standard approaches in speech recognition (and A.I. generally) rest on some very interesting assumptions that deserve to be carefully examined...

02 January 2021 9,363 11 View

Do children learn best when they're given the freedom to pursue their own interests?

21 October 2020 9,970 12 View

What sensors are applicable for plastic detection using Arduino?

Are there Arduino sensors that can be used to detect the presence of plastic materials? Our proposed study is an arduino based plastic detection from the water surface. We plan to use infrared...

16 October 2020 4,552 3 View

What is the ideal treatment of data for arduino-based research projects?

Our research is An Arduino-based solar-powered surface water plastic proximity detection and characterization system. We are currently writing our methodology for our research proposal but we are...

12 October 2020 905 3 View

C. S. Peirce held that, because mathematics makes no presuppositions, it is even more basic than phenomenology? Do you agree?

30 August 2020 3,686 13 View

Recovery rate of biodiversity when shifting from conventional to organic farming?

If it exists, where can I find data or some relevant references on the recovery rate (and time) of insect biodiversity (or just pollinators) when shifting from conventional to organic farming for...

11 August 2020 6,996 7 View

Wanulcas and banana - any experiences?

02 August 2020 1,358 3 View

What are the recommended diluents for the Pierce BCA protein assay kit?

I have performed a tissue homogenization/extraction protocol using methanol and chloroform as the solvents. The aliquot being using for BCA analysis contains both solvents. I understand that the...

23 June 2020 650 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View