What kind approaches I could try to study the protein's glycosylation? I want to know the weight of the glycan portion, there are any methods by mass espectrometry or eletrophoresis that can give me this information? Thanks!
Did you know the site of glycosylation? If you know the location of glycosylation at peptide level, you can get the the weight of the glycan portion by LC-MS of trypsin digested protein sample. Here is a reference paper: "Improved mass spectrometric characterization of protein glycosylation reveals unusual glycosylation of maize-derived bovine trypsin"(Analytical Chemistry 11/2010; 82(24):10095-101). If you do not know the site for glycosylation, you can do the LCMS w/o deglycosylation as mentioned by Nils. By this way you can locate the glycosylation site and get the weight.
There is a simple way: run the sample in a wide lane, transfer the proteins by western blotting onto a membrane, cut them into stripes and stain them with a specific antibody and a set of biotinylated lectins. This lectins recognize the terminal sugar residues specifically.
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