9 Questions 21 Answers 0 Followers
Questions related from Fernanda Amorim
Someone has an easy protocol for extraction of proteins from extracellular matrix? This material will be use for shotgun proteomics. Thanks =)
10 October 2017 5,987 0 View
I've been trying to do the linearization of the pPICZalfaA plasmid. The Easy Select kit (Invitrogen) says to use the restriction enzymes PmeI, SacI or BstXI. My insert does not have restriction...
05 May 2014 2,078 8 View
I need a protocol of colony PCR for confirmation of inserts after the Pichia pastoris electroporation. I performed a protocol, however, it didn't amplified any bands. So, I would like to compare...
05 May 2014 731 12 View
I am trying to cloning a protein in the pPICZalfaA vector, with the restriction enzyme SalI and XhoI, the colony PCR (with the specific forward primer for insert and reverse primer of vector) is...
02 February 2014 5,045 14 View
I need a software to analysis my contigs which I can edit them also. Which software I can use? I have the Codoncode, however, it's the demo mode version and I can't save the projects.
04 April 2013 7,486 11 View
What kind approaches I could try to study the protein's glycosylation? I want to know the weight of the glycan portion, there are any methods by mass espectrometry or eletrophoresis that can give...
03 March 2013 3,268 3 View
I think my protein could be a dimeric protein. What do I need to do to confirm this fact?
01 January 2013 4,541 50 View
Which sequence translated is more reliable?
11 November 2012 7,952 7 View
Is there any protocol or kit to do this?
11 November 2012 5,852 14 View
