Someone has an easy protocol for extraction of proteins from extracellular matrix? This material will be use for shotgun proteomics. Thanks =)
I need a protocol of colony PCR for confirmation of inserts after the Pichia pastoris electroporation. I performed a protocol, however, it didn't amplified any bands. So, I would like to compare...
04 May 2014 579 12 View
I've been trying to do the linearization of the pPICZalfaA plasmid. The Easy Select kit (Invitrogen) says to use the restriction enzymes PmeI, SacI or BstXI. My insert does not have restriction...
04 May 2014 2,040 8 View
I am trying to cloning a protein in the pPICZalfaA vector, with the restriction enzyme SalI and XhoI, the colony PCR (with the specific forward primer for insert and reverse primer of vector) is...
01 February 2014 4,961 14 View
I need a software to analysis my contigs which I can edit them also. Which software I can use? I have the Codoncode, however, it's the demo mode version and I can't save the projects.
03 April 2013 7,453 11 View
What kind approaches I could try to study the protein's glycosylation? I want to know the weight of the glycan portion, there are any methods by mass espectrometry or eletrophoresis that can give...
02 March 2013 3,215 3 View
I think my protein could be a dimeric protein. What do I need to do to confirm this fact?
31 December 2012 4,495 50 View
Is there any protocol or kit to do this?
10 November 2012 5,816 14 View
Which sequence translated is more reliable?
10 November 2012 7,897 7 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...
25 February 2021 1,660 3 View
Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...
25 February 2021 1,675 3 View
Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...
25 February 2021 3,612 3 View
Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...
24 February 2021 5,749 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....
22 February 2021 6,560 3 View
Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...
21 February 2021 7,447 2 View
Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...
17 February 2021 1,130 6 View
I have a 3D Image of size 100x100x100. I have features (feature dimension 25) extracted for each pixel by considering a small pacth around it. So the input to 1D CNN is 100x100x100x25 reshaped to...
17 February 2021 941 4 View