Someone has an easy protocol for extraction of proteins from extracellular matrix? This material will be use for shotgun proteomics. Thanks =)
I need a protocol of colony PCR for confirmation of inserts after the Pichia pastoris electroporation. I performed a protocol, however, it didn't amplified any bands. So, I would like to compare...
04 May 2014 760 12 View
I've been trying to do the linearization of the pPICZalfaA plasmid. The Easy Select kit (Invitrogen) says to use the restriction enzymes PmeI, SacI or BstXI. My insert does not have restriction...
04 May 2014 2,099 8 View
I am trying to cloning a protein in the pPICZalfaA vector, with the restriction enzyme SalI and XhoI, the colony PCR (with the specific forward primer for insert and reverse primer of vector) is...
01 February 2014 5,075 14 View
I need a software to analysis my contigs which I can edit them also. Which software I can use? I have the Codoncode, however, it's the demo mode version and I can't save the projects.
03 April 2013 7,508 11 View
What kind approaches I could try to study the protein's glycosylation? I want to know the weight of the glycan portion, there are any methods by mass espectrometry or eletrophoresis that can give...
02 March 2013 3,296 3 View
I think my protein could be a dimeric protein. What do I need to do to confirm this fact?
31 December 2012 4,574 50 View
Is there any protocol or kit to do this?
10 November 2012 5,874 14 View
Which sequence translated is more reliable?
10 November 2012 7,982 7 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
Is there a method that I can measure alcohol dehydrogenase activity using NAD+ in enzyme extraction from plants?
17 July 2024 935 1 View