Hello,

I have isolated exosomes from serum and plasma samples using the traditional ultracentrifugation and validated its size using the Nanosight. I would further want to validate its presence using the western blot. I am currently using TSG101 as an exosome marker. I have been estimating the concentration of a small volume of the whole exosome pellet using the bradford assay, and then took an aliquot needed to load 15-20 ug and treated that with RIPA buffer. Is this the right way to do this or should we lyse with RIPA buffer first and then estimate the concentration? Any suggestions would be highly helpful.

Thanks,