I have a given protease (with known sequence and structure). Is that possible to predict targets (with known sequence) through bioinformatics approaches?
You could see whether it is closely related to any other protease with known substrate specificity.
I am trying to put 3 separate plasmids into Staphylococcus aureus and I am looking for those that are theta replicating and compatible. I have obtained pSK9065 (from pSK1) from Addgene, but the...
23 July 2024 6,968 0 View
I am attempting to culture tissue resident T cells (TRM) derived from fresh intestinal tissue for up to six days. I am finding that the viability of the T cells is low at D0, and a very small...
08 July 2024 4,887 2 View
Hi all, Would anyone have any suggestions for articles and protocols for performing microdilution tests on candida biofilm? Do you recommend diluting the drug in the medium and adding it to the...
19 June 2024 4,420 3 View
I am trying to reconstitute the bacterium B.ovatus ATCC. it was first placed in a THIO broth and despite this we observed no turbidity. This was done 24h after the stick containing B.ovatus ATCC...
12 June 2024 1,644 3 View
How can you regulate the number of random inserted mutations in error prone PCR?
14 May 2024 673 2 View
Good evening, My IPA (Interpretative Phenomenological Analysis) research project involves conducting interviews in a different country and in a different language. Considering that I will need to...
05 May 2024 6,147 2 View
I am using this code (below) to use FDR to correct p values because I ran three exploratory growth curve models. How many p values do you need to correct? Do I just correct all of the p values for...
02 May 2024 4,028 4 View
We are interested in detecting aggrecan (250 KDa) by WB. No luck so far, are there any recommendations, such as running buffer, sample loading buffer? Thank you in advance!
05 April 2024 6,269 4 View
Hi :) How should I design primers for (-)ssRNA samples from Influenza A and hRSV viruses? I need to select the protein, locate the gene sequence, and then use it to design primers. Should I...
04 April 2024 4,896 1 View
Can I use feces preserved in formaldehyde to perform the McMaster technique for Haemonchus research?
20 March 2024 1,339 0 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View