What are the best references for an approach to teaching Differential Equations?

Differential equations are known to be potential tools for modeling and predicting recurrent phenomena in time and space, whether simple or more elaborate models. In this sense, how to approach...

18 December 2020 1,750 3 View

Best purification method for recombinant protein in BL21?

Hi I need to purify a recombinant protein (IL6 21kDa). It's been produced in E.coli BL21 with a T7 expression vector, but it does not have a tag as yet. I was thinking of adding a his-tag and...

09 December 2020 2,200 8 View

Recombinant Interleukin 6 western blot, non specific bands?

Hi I've been trying to produce some recombinant human Interleukin 6. I sub-cloned the gene for human IL6 into a Promega T7 Flexi vector (native gene no tag yet) and confirmed sequencing and...

09 December 2020 2,340 4 View

How many journal articles should come out of a doctoral thesis?

Is it normal if I publish my doctoral thesis into only one journal article (since my doctoral thesis will be data-heavy and theory-light)? Or are doctoral theses expected to be equivalent to 3-4...

01 December 2020 3,803 14 View

Can the potential of LIPIDMICS improve the diagnosis and understanding of metabolic changes in various neurodegenerative disease (PD, HD, AD, MS, etc?

08 November 2020 7,209 1 View

Can I compare data from ion exchange membranes with data from ion exchange resin when determining nutrient supply rates in soils?

Hi everyone, I am trying to decide if I can compare nutrient supply rates between two different methods of collection. Method A uses ion exchange membranes and method B uses ion exchange resin in...

23 September 2020 4,535 1 View

Why would a validation error (confusion) matrix return null in Google Earth Engine?

I am working on validating a collection of images from a time series in Google Earth Engine and the confusion matrix that results from validation of the trained image v.s. the landcover classes...

09 September 2020 7,246 3 View

How can I increase the length of DNA fragments recovered from QIAamp DNA Micro Kit?

Hi everyone. I'm performing DNA extractions for pools of ~10 individual Daphnia magna (water fleas) using the Qiagen QIAamp DNA Micro Kit. I use a pestle to homogenise the Daphnia before entering...

07 September 2020 9,956 1 View

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Nutritional science has provided functional foods with the required amounts of natural antioxidants, vitamins, fats, proteins, polyphenols, carbohydrates, and other components required to maintain...

03 June 2020 7,377 1 View

How do I choose an appropriate statistical test in SPSS?

03 May 2020 1,018 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to run raise regression in R?

if anybody know how to run raise regression in r, and raise parameter selection, please help me

24 February 2021 8,199 1 View

Can anyone suggest me the best way for Regression analysis of Accidents?

Dear Altruist, I want to make a regression analysis based on accident data. But I am not finding the necessary information about what type of regression is more applicable for analysis accident...

23 February 2021 9,956 3 View