We are trying to make the stable clone of SW480 colon cancer after transfecting with micro-plasmid containing GFP. At the beginning of transfection, GFP-expressing cells are a lot but in subsequent subcultures, with selection antibiotic (neomycin) treatment the GFP-expressing cells are going down and we lose them. How can we keep them growing without losing the transfected cells? 

Suggestions are well appreciated and thanks in advance...

Farhad

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