Dear all,
I am trying to get acquainted with "CCPN", a free NMR analysis package.
The software looks very nice and offers many features, however, it produces error after error when I am trying to do the tutorial and also sometimes crashes randomly.
I need to assign two-dimensional protein spectra (15N/proton HSQC) and read out the peak volumes - how can I get CCPN to run smoothly or are there better alternatives for the task I need to perform?
I am using PC-systems, maybe this is the problem, since CCPN seems to be written for MAC?
Thanks for any help,
regards,
Peter
Dear Peter,
I think CCPNMR analysis shouldn't have problem on Windows. Prof. Vuister said version 3 would come out soon, so the better version is on the dock hopefully.
If you cannot get it work with it, you can try NMRFAM-SPARKY as an alternative (or CARA or NMRView).
You can watch this video to see how it works.
https://youtu.be/gzcJA-fEko0
Extracting peak volume is extremely easy. Selecting peaks, integrate them (two-letter short-cut "pi") and in the peak list window (two-letter short-cut "lt"), Options -> check Volume -> click Ok.
You can download the software for free from http://pine.nmrfam.wisc.edu/download_packages.html
Best,
Woonghee
Thank you very much Woonghee for helping us out. I am just scanning through your publications, very impressive! How reliable is the peak volume determination algorithm in NMRFAM-SPARKY? Is the precision sufficient to extract kinetic data?
Sparky has been one of the most popular software packages for multidimensional NMR longer than a decade so it should be very reliable if your spectra are good. There are integration options- "Gaussian fit", "Lorentzian fit", "Sum over box" and "Sum over ellipse".
CcpNmr Analysis has a very helpful mailing list, at [email protected] and described at http://www.ccpn.ac.uk/v2-software/software/mailing
Send your questions there, and either a knowledgeable user or a developer will answer.
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