I use NanoDrop to check that I have reconstituted my peptide AB40 properly. My protocol is as follows

1)Open NanoDrop

2) Select Protien 280

3) Change Type to "Other Protien (EMW)

4) Enter (1.49) in sigma/1000 *Literature Value

5) Enter (4.33) in MW (kDa)

6) Type in Sample ID

7) Click Blank

8) Pipette 1.5uL of sample onto the NanoDrop

9) Click Measure

10) Repeat step 9 till concentration is constant

However I don't find NanoDrop as helpful when it comes to my buffer system. I too am looking for a good protocol for this as well. I'm told because I reconstitute my peptide with 60mM NaOH NanoDrop this is probably not as accurate as I'd like to believe it is.

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Dear Margarita,

Could I ask which kind of amyloid do you use and how do you prepare your amyloid to be aggregated? I bought amyloid 1-42 and want to use it to build Alzheimer disease model. I don not know how to prepare  it and make it have toxic to cell. Thanks....