Albert Pilkington

3 Questions 7 Answers 0 Followers

Questions related from Albert Pilkington

How does NH4OH or NaOH disaggregation protocol for Abeta actually break up existing beta sheet structures?

I understand the process behind why fluoro alcohols break up existing secondary structures, but for the alkaline conditions (NH4OH or NaOH) used in some protocols how are the basic conditions...

08 August 2015 8,223 9 View

Can anyone share Thioflavin T storage and oxidation conditions?

Okay this one is a pretty easy question, but I'm curious how other labs are storing their ThT salt. I get varying information from companies. We have been storing ours at RT in cabinet (bottle is...

08 August 2014 4,517 3 View

Can anyone help with my method for Tris buffer and Abeta fibrils?

Has anyone successfully seen Abeta fibrils in a Tris buffer within 72hrs? I'm currently doing ThT assays with 20uM Abeta40, Tris buffer, 20uM ThT and @ 37C for 72hrs. I'm not seeing anything and I...

06 June 2014 5,898 6 View