Hello,
I am using the Qiagen PCR clean-up and purification kit. I am purifying a PCR product of 750 bp.
I have added the buffer PB as described in the manual. The color of the mixture was violet (too high pH) so I added 10 microliters of 3M Sodium acetate (pH 5.0) as advised by the manual. However, the mixture remains pinkish in color.
is there any other way to regulate the pH and lower it?
Color of buffer-gel mixture was usually the result of dissolving a TAE or TBE agarose gel, to lower the pH can either decrease the size of excised gel or added with more sodium acetate. The goal is to lower the pH and facilitate the adsorption of DNA binding membrane.
Mu-Shen Chang I am using PCR product directly.
i kept added more acetate yet the color became a more diluted orange rather than yellow. (pH indicator gives yellow for pH 7.5)
Is there any other way to lower the pH?
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