M1, M2 macrophage markers
In a study done by Han Yu et al, 2016 demonstrate that CD206 and signal regulatory protein alpha (SIRPα) on macrophages were detected by flow cytometry in patient with MDs
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Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...
01 August 2024 476 2 View
I have a question about human macrophages cell surface markers.I want to stain the unpolarized human macrophages for flow cytometric sorting without permeabilizing them. It is because I need to...
29 July 2024 236 2 View
I have different polymer solutions and I hypothesize that water molecules are retained based on the water-polymer interactions, so the amount of free water is less to intercat with drug tablet....
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Hi, I need help in detaching adherent monocytes from well plates. I isolated the PBMCs from buffy coat and I did CD14+ positive selection to collect only the monocytes. I let them adhere on my...
20 July 2024 7,922 2 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
Recently, I tried to differentiate THP1 into M2 macrophage using IL4 and IL13 (purchased from R&D). This is my protocol. THP1 was seeded into 6-well plate (10E6 cells/well). Incubate with PMA...
15 July 2024 5,153 2 View
Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...
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I am a beginner with ImageJ and would like to know how to quantify fluorescence images of platelets taken with a fluorescence microscope at 40x and 100x magnification. If you could provide...
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