PLGA nanoparticles containing HIP complex of drug+surfactant (size 150 nm) from non entrapped complex (size 250nm) for determination of encapsulation efficieny, can I separate them by dialysis?
These particles are too large for dialysis. Sucrose, glycerol, or Percoll density gradient centrifugation might work if the two types of particles differ significantly in their buoyant densities.
Adam B Shapiro
can i add Nacl in the dialysate to solubilize the complex without affecting nps?
I think ultracentrifyge will precepitate both the Nps and the complex.
V. Andrés-Guerrero
Thank you so much i will try them.
really appreciate your help
Filter size cutoffs are typically not sharp enough to ensure that you will get a good separation of 150 nm and 250 nm particles with a 0.22 micron filter. Particle sizes distributions are also not typically that narrow.
I agree with Dr Shapiro: by using microfiltration you can hardly get full separation, and not only because of overlapping of the particle fractions; the microfilter is probable to be blinded soon. Perrhaps you could try gel filtration by smth like Sephacryl in long column (20-30 cm or longer, the peaks must be apart ). In any case, please inform in how you managed to solve a problem
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