Problem with minigels that are leaking?

More Sanda Mimouna's questions See All

Is this the way one computes macro-accuracy for BERT multi-classification text?

The code is: import evaluate metric = evaluate.load("accuracy") def compute_metrics(eval_pred): logits, labels = eval_pred predictions = np.argmax(logits, axis=-1) return...

28 December 2022 2,432 3 View

Why there are other results with the same seed for Transformers?

I have: from transformers import TrainingArguments, Trainer training_args = TrainingArguments(output_dir=“testTrainer”, evaluation_strategy=“epoch”, seed=42, num_train_epochs=10.0,...

17 December 2022 7,096 1 View

Is Hugging Face's evaluate-metric/accuracy the same as macro-accuracy?

I am running tests on BERT transformers and using the evaluate Python library. On their site, it says: "computed with Accuracy = (TP + TN) / (TP + TN + FP + FN) Where: TP: True positive TN: True...

10 December 2022 7,339 2 View

Does anyone know of applications of Benford's law on text?

I know that research has been done for using Benford's Law on images. I am wondering if anyone tried to do the same on text.

24 August 2022 4,713 1 View

How can I obtain the Eneggy spectrum of particules simulated with EPOCH code, using MATLAB?

I wanna to have proton energy according to their number ( E=f(N) ).

23 January 2021 2,975 2 View

Can anyone give me the best conditions to expand CD4 T cells from spleen an LN of mice for passive EAE?

I am inducing EAE in mice by adoptive transfer from female donor mice. The Hoocke protocol I follow states that I should be able to harvest enough CD4 Th1 from each donor mouse to inject in 3...

09 December 2017 335 0 View

Huge vacuoles in fibroblasts?

Hi everybody! Do you know what these vacuoles could be? The cells are primary human skin fibroblasts treated with molecule. Cells do not die from this treatment. Thank you for your help

19 March 2017 9,079 5 View

Could anyone please tell me how can I use a previously calculated solution in 1st geometry to new geometry model in COMSOL?

Currently I'm working on a COMSOL project on geomechanics and that have two physics models of solid mechanics. And i am changing geometry by making holes in horizontal direction (representing...

17 November 2016 10,024 2 View

Does anyone know a free data base with the lakes from India (shapefile type) ??

I need water bodies from India, shapefile type, with the name in the atribute table.

21 March 2016 5,455 5 View

What is the best antibody to detect MUC2 on a western blot of mice stools?

10 March 2015 6,091 2 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

Why my gel electrophoresis has smearing?

Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...

22 July 2024 9,761 5 View

How should we increase the quality of RNA extraction?

I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...

22 July 2024 155 4 View

Jose L Chavez

when do you have the leaking problem?

Is this happening when you are setting the gel?

What I normally do is to dry very well with towel paper the gel and the cast before to put the gel in the system.

Brandon A Kemp

have you checked your green rubber seals? Are they worn or dry rotted? Are they flush with the apparatus? This happened to me and when I purchased a new seal, it stopped leaking.