What is the best antibody to detect MUC2 on a western blot of mice stools?

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Is this the way one computes macro-accuracy for BERT multi-classification text?

The code is: import evaluate metric = evaluate.load("accuracy") def compute_metrics(eval_pred): logits, labels = eval_pred predictions = np.argmax(logits, axis=-1) return...

28 December 2022 2,432 3 View

Why there are other results with the same seed for Transformers?

I have: from transformers import TrainingArguments, Trainer training_args = TrainingArguments(output_dir=“testTrainer”, evaluation_strategy=“epoch”, seed=42, num_train_epochs=10.0,...

17 December 2022 7,096 1 View

Is Hugging Face's evaluate-metric/accuracy the same as macro-accuracy?

I am running tests on BERT transformers and using the evaluate Python library. On their site, it says: "computed with Accuracy = (TP + TN) / (TP + TN + FP + FN) Where: TP: True positive TN: True...

10 December 2022 7,339 2 View

Does anyone know of applications of Benford's law on text?

I know that research has been done for using Benford's Law on images. I am wondering if anyone tried to do the same on text.

24 August 2022 4,713 1 View

How can I obtain the Eneggy spectrum of particules simulated with EPOCH code, using MATLAB?

I wanna to have proton energy according to their number ( E=f(N) ).

23 January 2021 2,975 2 View

Can anyone give me the best conditions to expand CD4 T cells from spleen an LN of mice for passive EAE?

I am inducing EAE in mice by adoptive transfer from female donor mice. The Hoocke protocol I follow states that I should be able to harvest enough CD4 Th1 from each donor mouse to inject in 3...

09 December 2017 335 0 View

Huge vacuoles in fibroblasts?

Hi everybody! Do you know what these vacuoles could be? The cells are primary human skin fibroblasts treated with molecule. Cells do not die from this treatment. Thank you for your help

19 March 2017 9,079 5 View

Could anyone please tell me how can I use a previously calculated solution in 1st geometry to new geometry model in COMSOL?

Currently I'm working on a COMSOL project on geomechanics and that have two physics models of solid mechanics. And i am changing geometry by making holes in horizontal direction (representing...

17 November 2016 10,024 2 View

Does anyone know a free data base with the lakes from India (shapefile type) ??

I need water bodies from India, shapefile type, with the name in the atribute table.

21 March 2016 5,455 5 View

Problem with minigels that are leaking?

Hey is someone using the Ready Gel Cell kit from Bio Rad? if yes how do you stop them from leaking please? thank you

02 March 2015 3,631 2 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

How to determine method detection limit in an analytical method?

I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...

24 July 2024 6,592 5 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View