Hi could anyone point me in the direction of a paper that talks about the length of time that mature (i.e. not BMDC) DCs eg from lymph nodes can be incubated ex vivo before cell death?
For mature dendritic cells (CD208 / CDLamp +) isolated from Thymus, tonsil or lung, the duration of live does not exceed 24 hours without the addition of cytokines and vital stimuli for their in vitro culture (ie: IL4 , GM-CSF, Il10 and CD40 interaction)