Eleanor Sherwood

2 Questions 1 Answers 0 Followers

Questions related from Eleanor Sherwood

Primary dendritic cell culture?

Hi could anyone point me in the direction of a paper that talks about the length of time that mature (i.e. not BMDC) DCs eg from lymph nodes can be incubated ex vivo before cell death?

13 November 2017 4,820 2 View

How to get the best quality and concentration RNA from primary cells?

I am currently using Qiagen micro/mini RNeasy extraction kits to extract RNA from primary mouse DCs following 6 hour incubation in culture media in order to perform RNA sequencing. However,...

24 July 2015 9,304 2 View